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This procedure enables the study of the intestinal digestion, absorption and lymphatic transport of dietary lipid in the conscious mouse. By infusing the lipid test meal intraduadenally at a constant rate, the lymphatic lipid output usually reaches a steady rate by the 3rd or 4th hour. Thus, lymphatic lipid output during the 5th or 6th hour represents the amount of lipid transported by the small intestine under steady-state conditions. At the end of the study, both luminal as well as mucosal radioactive lipids can be collected. Thus one can obtain a considerable amount of information regarding the digestion, uptake and mucosal esterification and the lymphatic transport of lipids.
Determinations of substrates in plasma/serum/lymph will be made using specific colorimetric assays. Reactions will be run in microtiter plates and analyzed on a plate reader. Using this method assays can be performed on small (5-25ul) samples of plasma or serum.
Cholesterol is measured in lipid extracted via Folch extraction from tissue. This service is completed in conjunction with C1104*.
UC Reading CampusMetabolic Diseases Institute2120 E. Galbraith Rd.Cincinnati, OH 45237Phone: 513-558-2748