Today is Sunday, Jul. 23, 2017

Department of

Pharmacology & Systems Physiology 

Department Resources

Zeiss LSM 710 LIVE Duo Confocal Microscope

Go to Microscope Scheduler(UC or VPN access only)

  • Available 24/7
  • Equipped with two separate scanning units, the fast-speed line (LIVE) scanner (up to 120 frames/sec at 512x512) and the LSM710 high-resolution point scanner, integrated into the same microscope.
  • Point Scanner: 6 laser lines (405, 458, 488, 514, 560, 633nm) and 4 detectors (3 spectral confocal fluorescence plus 1 transmitted light detector).
  • LIVE fast line scanner: 3 laser lines (405, 488, 560nm) and 2 confocal fluorescence detectors.
  • The Duo system allows the two scanners to be used in combination for complex, high-speed bleaching or photomanipulation experiments.
  • Temperature-controlled environmental chamber encasing entire microscope. 

Zeiss LSM 510 NLO Confocal and Two-Photon Microscope

Go to LCM Scheduler(UC or VPN access only)
  • Available 24/7
  • 4 lasers (Ti-Sa [700-990 nm; wavelength selection under computer control], Ar [458,477,488,514 nm], Green HeNe [543 nm] and Red HeNe [633 nm])
  • 6 detectors [3 confocal fluorescence/reflectance, 1 Nomarski transmitted, 2 non-descanned high sensitivity two-photon detectors]).
  • Useful for deep tissue imaging (better than standard confocal) while retaining subcellular resolution in living tissue.
  • The two-photon absorption also allows fluorophore uncaging, cell damage/ablation, spectral shifting, and other photo-catalytic events to be performed with sub-micron resolution in three dimensions.
  • Two-photon and confocal microscope.
  • Microscope: Zeiss Axiovert 200 inverted microscope platform.
  • Dark Environmental chamber: encloses microscope to allow 37C control and keep stray light out of detectors.
  • Microscope objectives: 40x C-Apochromat (water immersion), 10x C-Apochromat (water immersion)
  • Fluorescence filter blocks for direct visualization of double and triple labeled samples by eye

Arcturus (Life Technologies) XT Laser Capture Microdissection microscope. 

Go to LCM Scheduler(UC or VPN access only)
  • Available 24/7.
  • Automated microdissection of individual cells or multicellular structures from slides containing tissue sections or cytological samples.
  • Harvest single cells or tens of thousands of cells in seconds.
  • Suitable for RNA, DNA, and/or protein analysis. 
  • Up to three slides can mounted simultaneously, and regions to be collected can be defined under transmitted light imaging and/or fluorescence imaging.
  • IR Laser Capture and UV Laser Cutting
  • Epi-fluorescence (1500-hr Metal Halide lamp)
  • Microscope Objectives: 2x, 10x, 20x, and 60x Nikon Infinity-corrected objectives
  • Motorized stage with positions for 3 slides and 8 caps

Leica DMi8 Widefield Live-Imaging Microscope.

  • Widefield microscope system equipped with a stage-top incubation chamber for live time-lapse imaging experiments in brightfield and fluorescence.
  • Color camera for histology and phase contrast imaging, and high-sensitivity Hamamatsu ORCA-ER cooled CCD camera for fluorescence imaging.
  • Sola LED light source and 4 fluorescence filters (DAPI, GFP, Texas Red, CY5).
  • Motorized stage enables image stitching of large regions at high magnification.

Leica MZ16FA Stereo Zoom Fluorescence microscope.

  • Continuous zoom from 7x – 230x total magnification.
  • Host computer drives 12-bit color camera to capture digital images in bright field, dark field, or fluorescence.  
  • Filters for UV and GFP fluorescence excitation, or can accept user-defined filters.  
  • Stereo microscopy allows viewing specimens three-dimensionally, with great depth of field, in large fields of view.

Centrifuges / Ultracentrifuges

  • Ultracentrifuges
  • Medium-Speed Centrifuges
  • Low-Speed Centrifuges

Cryostat - Leica CM 1900

  • Microtome encapsulated in a cryochamber to cut frozen tissue sections up to 60 micrometers thick
  • Includes separate specimen cooling system, independent of chamber cooling, both adjustable from 0 to -35 degrees Celsius.

 

MCP Room Schedules

To make a reservation, please contact Cheryl Holly, Tel 8-5636, or Jeannie Cummins, Tel 8-3102.

CARE/Crawley Building

  • CARE 1819
  • CARE 1870
  • CARE 4819

Medical Sciences Building

  • MSB 4104 (Lamport Library)
  • MSB 4206